Construct: shRNA TRCN0000429300

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_010957.4-1371s21c1
DNA Barcode:
CCTAAGTCTCAAGTCAGAAAG

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Ogg1 (18294)
Gene Description:
8-oxoguanine DNA-glycosylase 1
Transcript:
RefSeq NM_010957.4 (CURRENT)
Match location:
Position 1371 (3UTR)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000429300)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCTAAGTCTCAAGTCAGAAAG
Hairpin Sequence:
5'-CCGG-CCTAAGTCTCAAGTCAGAAAG-CTCGAG-CTTTCTGACTTGAGACTTAGG-TTTTTTGAAT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCTAAGTCTCAAGTCAGAAAGCTCGAGCTTTCTGACTTGAGACTTAGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCTAAGTCTCAAGTCAGAAAGCTCGAGCTTTCTGACTTGAGACTTAGG-3'

Other clones with same target sequence:

(none)