Construct: shRNA TRCN0000429306

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_028829.3-1496s21c1
DNA Barcode:
CATTGGTAGAGAGTGCTTATA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Paqr8 (74229)
Gene Description:
progestin and adipoQ receptor family member VIII
Transcript:
RefSeq NM_028829.3 (CURRENT)
Match location:
Position 1496 (3UTR)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000429306)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CATTGGTAGAGAGTGCTTATA
Hairpin Sequence:
5'-CCGG-CATTGGTAGAGAGTGCTTATA-CTCGAG-TATAAGCACTCTCTACCAATG-TTTTTTGAAT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCATTGGTAGAGAGTGCTTATACTCGAGTATAAGCACTCTCTACCAATGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACATTGGTAGAGAGTGCTTATACTCGAGTATAAGCACTCTCTACCAATG-3'

Other clones with same target sequence:

(none)