Construct: shRNA TRCN0000429334

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_014404.1-518s21c1
DNA Barcode:
ACAAGTATGGGTGGTCGTTTG

Original Target:

Taxon:
Homo sapiens (human)
Gene:
CACNG5 (27091)
Gene Description:
calcium voltage-gated channel auxiliary subunit gamma 5
Transcript:
RefSeq NM_014404.1 (NON-CURRENT)
Match location:
Position 518 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000429334)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
ACAAGTATGGGTGGTCGTTTG
Hairpin Sequence:
5'-CCGG-ACAAGTATGGGTGGTCGTTTG-CTCGAG-CAAACGACCACCCATACTTGT-TTTTTTGAAT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGACAAGTATGGGTGGTCGTTTGCTCGAGCAAACGACCACCCATACTTGTTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAACAAGTATGGGTGGTCGTTTGCTCGAGCAAACGACCACCCATACTTGT-3'

Other clones with same target sequence:

(none)