Construct: shRNA TRCN0000429374

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_144896.4-506s21c1
DNA Barcode:
ACTTGACCTACTGTATCTATC

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Gatb (229487)
Gene Description:
glutamyl-tRNA(Gln) amidotransferase, subunit B
Transcript:
RefSeq NM_144896.4 (CURRENT)
Match location:
Position 506 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000429374)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
ACTTGACCTACTGTATCTATC
Hairpin Sequence:
5'-CCGG-ACTTGACCTACTGTATCTATC-CTCGAG-GATAGATACAGTAGGTCAAGT-TTTTTTGAAT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGACTTGACCTACTGTATCTATCCTCGAGGATAGATACAGTAGGTCAAGTTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAACTTGACCTACTGTATCTATCCTCGAGGATAGATACAGTAGGTCAAGT-3'

Other clones with same target sequence:

(none)