Construct: shRNA TRCN0000429375

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_178527.3-2309s21c1
DNA Barcode:
GGCCAATGGCAAGAGGTTTAA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
SLC9C2 (284525)
Gene Description:
solute carrier family 9 member C2 (putative)
Transcript:
RefSeq NM_178527.3 (NON-CURRENT)
Match location:
Position 2309 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000429375)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GGCCAATGGCAAGAGGTTTAA
Hairpin Sequence:
5'-CCGG-GGCCAATGGCAAGAGGTTTAA-CTCGAG-TTAAACCTCTTGCCATTGGCC-TTTTTTGAAT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGGCCAATGGCAAGAGGTTTAACTCGAGTTAAACCTCTTGCCATTGGCCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGGCCAATGGCAAGAGGTTTAACTCGAGTTAAACCTCTTGCCATTGGCC-3'

Other clones with same target sequence:

(none)