Construct: shRNA TRCN0000429415

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_021907.3-554s21c1
DNA Barcode:
ATTAGTGTGGAACAATCTATC

Original Target:

Taxon:
Homo sapiens (human)
Gene:
DTNB (1838)
Gene Description:
dystrobrevin beta
Transcript:
RefSeq NM_021907.3 (NON-CURRENT)
Match location:
Position 554 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000429415)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
ATTAGTGTGGAACAATCTATC
Hairpin Sequence:
5'-CCGC-ATTAGTGTGGAACAATCTATC-CTCGAG-GATAGATTGTTCCACACTAAT-TTTTTTGAAT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGATTAGTGTGGAACAATCTATCCTCGAGGATAGATTGTTCCACACTAATTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAATTAGTGTGGAACAATCTATCCTCGAGGATAGATTGTTCCACACTAAT-3'

Other clones with same target sequence:

(none)