Construct: shRNA TRCN0000429485

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_017610.6-2866s21c1
DNA Barcode:
GAGTGACTGCAGCTACTTATA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
RNF111 (54778)
Gene Description:
ring finger protein 111
Transcript:
RefSeq NM_017610.6 (NON-CURRENT)
Match location:
Position 2866 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000429485)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GAGTGACTGCAGCTACTTATA
Hairpin Sequence:
5'-CCGG-GAGTGACTGCAGCTACTTATA-CTCGAG-TATAAGTAGCTGCAGTCACTC-TTTTTTGAAT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGAGTGACTGCAGCTACTTATACTCGAGTATAAGTAGCTGCAGTCACTCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGAGTGACTGCAGCTACTTATACTCGAGTATAAGTAGCTGCAGTCACTC-3'

Other clones with same target sequence:

(none)