Construct: shRNA TRCN0000429543

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_000890.3-2058s21c1
DNA Barcode:
AGATCCATCCATATCACATAG

Original Target:

Taxon:
Homo sapiens (human)
Gene:
KCNJ5 (3762)
Gene Description:
potassium inwardly rectifying channel subfamily J member 5
Transcript:
RefSeq NM_000890.3 (NON-CURRENT)
Match location:
Position 2058 (3UTR)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000429543)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
AGATCCATCCATATCACATAG
Hairpin Sequence:
5'-CCGG-AGATCCATCCATATCACATAG-CTCGAG-CTATGTGATATGGATGGATCT-TTTTTTGAAT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGAGATCCATCCATATCACATAGCTCGAGCTATGTGATATGGATGGATCTTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAAGATCCATCCATATCACATAGCTCGAGCTATGTGATATGGATGGATCT-3'

Other clones with same target sequence:

(none)