Construct: shRNA TRCN0000429545

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_173647.3-1360s21c1
DNA Barcode:
AGAAGTTTGGCTTGAACTAAA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
RNF149 (284996)
Gene Description:
ring finger protein 149
Transcript:
RefSeq NM_173647.3 (NON-CURRENT)
Match location:
Position 1360 (3UTR)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000429545)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
AGAAGTTTGGCTTGAACTAAA
Hairpin Sequence:
5'-CCGG-AGAAGTTTGGCTTGAACTAAA-CTCGAG-TTTAGTTCAAGCCAAACTTCT-TTTTTTTGAT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGAGAAGTTTGGCTTGAACTAAACTCGAGTTTAGTTCAAGCCAAACTTCTTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAAGAAGTTTGGCTTGAACTAAACTCGAGTTTAGTTCAAGCCAAACTTCT-3'

Other clones with same target sequence:

(none)