Construct: shRNA TRCN0000429545
Construct Description:
- Construct Type:
- shRNA
- Other Identifiers:
- NM_173647.3-1360s21c1
- DNA Barcode:
- AGAAGTTTGGCTTGAACTAAA
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- RNF149 (284996)
- Gene Description:
- ring finger protein 149
- Transcript:
- RefSeq NM_173647.3 (NON-CURRENT)
- Match location:
- Position 1360 (3UTR)
Vector Information:
- Vector Backbone:
- pLKO_005
- Pol II Cassette 1:
- PGK-PuroR
- Pol II Cassette 2:
- n/a
- Pol III Promoter:
- constitutive hU6
- Pol III Insert:
- (TRCN0000429545)
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
Current transcripts matched by this shRNA:
Sequence Information
- Target Sequence:
- AGAAGTTTGGCTTGAACTAAA
- Hairpin Sequence:
-
5'-CCGG-AGAAGTTTGGCTTGAACTAAA-CTCGAG-TTTAGTTCAAGCCAAACTTCT-TTTTTTTGAT-3'
Oligo design for arrayed cloning:
- Forward sequence:
- 5'-CCGGAGAAGTTTGGCTTGAACTAAACTCGAGTTTAGTTCAAGCCAAACTTCTTTTTTG-3'
- Reverse sequence:
- 5'-AATTCAAAAAAGAAGTTTGGCTTGAACTAAACTCGAGTTTAGTTCAAGCCAAACTTCT-3'
Other clones with same target sequence:
(none)
