Construct: shRNA TRCN0000429547

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_017416.1-2324s21c1
DNA Barcode:
CACTAAAGCGTAGCATCAAAC

Original Target:

Taxon:
Homo sapiens (human)
Gene:
IL1RAPL2 (26280)
Gene Description:
interleukin 1 receptor accessory protein like 2
Transcript:
RefSeq NM_017416.1 (NON-CURRENT)
Match location:
Position 2324 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000429547)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CACTAAAGCGTAGCATCAAAC
Hairpin Sequence:
5'-CCGG-CACTAAAGCGTAGCATCAAAC-CTCGAG-GTTTGATGCTACGCTTTAGTG-TTTTTTGAAT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCACTAAAGCGTAGCATCAAACCTCGAGGTTTGATGCTACGCTTTAGTGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACACTAAAGCGTAGCATCAAACCTCGAGGTTTGATGCTACGCTTTAGTG-3'

Other clones with same target sequence:

(none)