Construct: shRNA TRCN0000429560

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_054039.1-1851s21c1
DNA Barcode:
ACAGACACCATCCTAATATTT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Foxp3 (20371)
Gene Description:
forkhead box P3
Transcript:
RefSeq NM_054039.1 (NON-CURRENT)
Match location:
Position 1851 (3UTR)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000429560)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
ACAGACACCATCCTAATATTT
Hairpin Sequence:
5'-CCGG-ACAGACACCATCCTAATATTT-CTCGAG-AAATATTAGGATGGTGTCTGT-TTTTTTGAAT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGACAGACACCATCCTAATATTTCTCGAGAAATATTAGGATGGTGTCTGTTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAACAGACACCATCCTAATATTTCTCGAGAAATATTAGGATGGTGTCTGT-3'

Other clones with same target sequence:

(none)