Construct: shRNA TRCN0000429594

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_000168.5-1575s21c1
DNA Barcode:
AGCAGCTTGTGCACCATATAA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
GLI3 (2737)
Gene Description:
GLI family zinc finger 3
Transcript:
RefSeq NM_000168.5 (NON-CURRENT)
Match location:
Position 1575 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000429594)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
AGCAGCTTGTGCACCATATAA
Hairpin Sequence:
5'-CCGG-AGCAGCTTGTGCACCATATAA-CTCGAG-TTATATGGTGCACAAGCTGCT-TTTTTTGAAT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGAGCAGCTTGTGCACCATATAACTCGAGTTATATGGTGCACAAGCTGCTTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAAGCAGCTTGTGCACCATATAACTCGAGTTATATGGTGCACAAGCTGCT-3'

Other clones with same target sequence:

(none)