Construct: shRNA TRCN0000429601

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_144873.2-2586s21c1
DNA Barcode:
CATGATCTCGGGCAGAATTAT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Uhrf2 (109113)
Gene Description:
ubiquitin-like, containing PHD and RING finger domains 2
Transcript:
RefSeq NM_144873.2 (CURRENT)
Match location:
Position 2586 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000429601)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CATGATCTCGGGCAGAATTAT
Hairpin Sequence:
5'-CCGG-CATGATCTCGGGCAGAATTAT-CTCGAG-ATAATTCTGCCCGAGATCATG-TTTTTTGAAT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCATGATCTCGGGCAGAATTATCTCGAGATAATTCTGCCCGAGATCATGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACATGATCTCGGGCAGAATTATCTCGAGATAATTCTGCCCGAGATCATG-3'

Other clones with same target sequence:

(none)