Construct: shRNA TRCN0000429642

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_173514.2-828s21c1
DNA Barcode:
CATTGGAGCAATGATAGTTTA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
SLC38A9 (153129)
Gene Description:
solute carrier family 38 member 9
Transcript:
RefSeq NM_173514.2 (NON-CURRENT)
Match location:
Position 828 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000429642)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CATTGGAGCAATGATAGTTTA
Hairpin Sequence:
5'-CCGG-CATTGGAGCAATGATAGTTTA-CTCGAG-TAAACTATCATTGCTCCAATG-TTTTTTGAAT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCATTGGAGCAATGATAGTTTACTCGAGTAAACTATCATTGCTCCAATGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACATTGGAGCAATGATAGTTTACTCGAGTAAACTATCATTGCTCCAATG-3'

Other clones with same target sequence:

(none)