Construct: shRNA TRCN0000429726

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_002349.2-5516s21c1
DNA Barcode:
GTGATTGGTAACTAGGATATG

Original Target:

Taxon:
Homo sapiens (human)
Gene:
LY75 (4065)
Gene Description:
lymphocyte antigen 75
Transcript:
RefSeq NM_002349.2 (NON-CURRENT)
Match location:
Position 5516 (3UTR)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000429726)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GTGATTGGTAACTAGGATATG
Hairpin Sequence:
5'-CCGG-GTGATTGGTAACTAGGATATG-CTCGAG-CATATCCTAGTTACCAATCAC-TTTTTTGAAT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGTGATTGGTAACTAGGATATGCTCGAGCATATCCTAGTTACCAATCACTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGTGATTGGTAACTAGGATATGCTCGAGCATATCCTAGTTACCAATCAC-3'

Other clones with same target sequence:

(none)