Construct: shRNA TRCN0000429758

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_011537.3-1195s21c1
DNA Barcode:
CCCGATTACACATCGTGAAAG

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Tbx5 (21388)
Gene Description:
T-box 5
Transcript:
RefSeq NM_011537.3 (CURRENT)
Match location:
Position 1195 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000429758)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCCGATTACACATCGTGAAAG
Hairpin Sequence:
5'-CCGG-CCCGATTACACATCGTGAAAG-CTCGAG-CTTTCACGATGTGTAATCGGG-TTTTTTGAAT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCCGATTACACATCGTGAAAGCTCGAGCTTTCACGATGTGTAATCGGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCCGATTACACATCGTGAAAGCTCGAGCTTTCACGATGTGTAATCGGG-3'

Other clones with same target sequence:

(none)