Construct: shRNA TRCN0000429763

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_005379.2-2627s21c1
DNA Barcode:
TGCCATCCACAAACGTCTTAG

Original Target:

Taxon:
Homo sapiens (human)
Gene:
MYO1A (4640)
Gene Description:
myosin IA
Transcript:
RefSeq NM_005379.2 (NON-CURRENT)
Match location:
Position 2627 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000429763)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
TGCCATCCACAAACGTCTTAG
Hairpin Sequence:
5'-CCGG-TGCCATCCACAAACGTCTTAG-CTCGAG-CTAAGACGTTTGTGGATGGCA-TTTTTTGAAT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGTGCCATCCACAAACGTCTTAGCTCGAGCTAAGACGTTTGTGGATGGCATTTTTG-3'
Reverse sequence:
5'-AATTCAAAAATGCCATCCACAAACGTCTTAGCTCGAGCTAAGACGTTTGTGGATGGCA-3'

Other clones with same target sequence:

(none)