Construct: shRNA TRCN0000429774

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_010957.4-1001s21c1
DNA Barcode:
GCTGACTGCATCTGCTTAATG

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Ogg1 (18294)
Gene Description:
8-oxoguanine DNA-glycosylase 1
Transcript:
RefSeq NM_010957.4 (CURRENT)
Match location:
Position 1001 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000429774)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCTGACTGCATCTGCTTAATG
Hairpin Sequence:
5'-CCGG-GCTGACTGCATCTGCTTAATG-CTCGAG-CATTAAGCAGATGCAGTCAGC-TTTTTTGAAT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCTGACTGCATCTGCTTAATGCTCGAGCATTAAGCAGATGCAGTCAGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCTGACTGCATCTGCTTAATGCTCGAGCATTAAGCAGATGCAGTCAGC-3'

Other clones with same target sequence:

(none)