Construct: shRNA TRCN0000429878

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_205849.2-851s21c1
DNA Barcode:
ACTCGTCTGTTAAATGCCAGA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
FAM9B (171483)
Gene Description:
family with sequence similarity 9 member B
Transcript:
RefSeq NM_205849.2 (NON-CURRENT)
Match location:
Position 851 (3UTR)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000429878)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
ACTCGTCTGTTAAATGCCAGA
Hairpin Sequence:
5'-CCGG-ACTCGTCTGTTAAATGCCAGA-CTCGAG-TCTGGCATTTAACAGACGAGT-TTTTTTGAAT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGACTCGTCTGTTAAATGCCAGACTCGAGTCTGGCATTTAACAGACGAGTTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAACTCGTCTGTTAAATGCCAGACTCGAGTCTGGCATTTAACAGACGAGT-3'

Other clones with same target sequence:

(none)