Construct: shRNA TRCN0000429887

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_000816.3-614s21c1
DNA Barcode:
GGAGTGAAGCCAACGTTAATT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
GABRG2 (2566)
Gene Description:
gamma-aminobutyric acid type A receptor gamma2 subunit
Transcript:
RefSeq NM_000816.3 (CURRENT)
Match location:
Position 614 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000429887)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GGAGTGAAGCCAACGTTAATT
Hairpin Sequence:
5'-CCGG-GGAGTGAAGCCAACGTTAATT-CTCGAG-AATTAACGTTGGCTTCACTCC-TTTTTTGAAT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGGAGTGAAGCCAACGTTAATTCTCGAGAATTAACGTTGGCTTCACTCCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGGAGTGAAGCCAACGTTAATTCTCGAGAATTAACGTTGGCTTCACTCC-3'

Other clones with same target sequence:

(none)