Construct: shRNA TRCN0000429918

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_021980.4-318s21c1
DNA Barcode:
ACCCAAACCTGGACACGTTTA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
OPTN (10133)
Gene Description:
optineurin
Transcript:
RefSeq NM_021980.4 (CURRENT)
Match location:
Position 318 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000429918)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
ACCCAAACCTGGACACGTTTA
Hairpin Sequence:
5'-CCGG-ACCCAAACCTGGACACGTTTA-CTCGAG-TAAACGTGTCCAGGTTTGGGT-TTTTTTGAAT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGACCCAAACCTGGACACGTTTACTCGAGTAAACGTGTCCAGGTTTGGGTTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAACCCAAACCTGGACACGTTTACTCGAGTAAACGTGTCCAGGTTTGGGT-3'

Other clones with same target sequence:

(none)