Construct: shRNA TRCN0000429927

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_145266.4-304s21c1
DNA Barcode:
GATGAGGGAACATGGACTTTG

Original Target:

Taxon:
Homo sapiens (human)
Gene:
NUDCD2 (134492)
Gene Description:
NudC domain containing 2
Transcript:
RefSeq NM_145266.4 (NON-CURRENT)
Match location:
Position 304 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000429927)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GATGAGGGAACATGGACTTTG
Hairpin Sequence:
5'-CCGG-GATGAGGGAACATGGACTTTG-CTCGAG-CAAAGTCCATGTTCCCTCATC-TTTTTTGAAT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGATGAGGGAACATGGACTTTGCTCGAGCAAAGTCCATGTTCCCTCATCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGATGAGGGAACATGGACTTTGCTCGAGCAAAGTCCATGTTCCCTCATC-3'

Other clones with same target sequence:

(none)