Construct: shRNA TRCN0000429980

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_173685.2-412s21c1
DNA Barcode:
GACAAGGCAATGGTTGAATTT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
NSMCE2 (286053)
Gene Description:
NSE2 (MMS21) homolog, SMC5-SMC6 complex SUMO ligase
Transcript:
RefSeq NM_173685.2 (NON-CURRENT)
Match location:
Position 412 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000429980)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GACAAGGCAATGGTTGAATTT
Hairpin Sequence:
5'-CCGG-GACAAGGCAATGGTTGAATTT-CTCGAG-AAATTCAACCATTGCCTTGTC-TTTTTTGAAT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGACAAGGCAATGGTTGAATTTCTCGAGAAATTCAACCATTGCCTTGTCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGACAAGGCAATGGTTGAATTTCTCGAGAAATTCAACCATTGCCTTGTC-3'

Other clones with same target sequence:

(none)