Construct: shRNA TRCN0000429988

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_026536.1-703s21c1
DNA Barcode:
TGATACTAGACAGCTACAAAT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Atp5s (68055)
Gene Description:
ATP synthase, H+ transporting, mitochondrial F0 complex, subunit S
Transcript:
RefSeq NM_026536.1 (CURRENT)
Match location:
Position 703 (3UTR)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000429988)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
TGATACTAGACAGCTACAAAT
Hairpin Sequence:
5'-CCGG-TGATACTAGACAGCTACAAAT-CTCGAG-ATTTGTAGCTGTCTAGTATCA-TTTTTTGAAT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGTGATACTAGACAGCTACAAATCTCGAGATTTGTAGCTGTCTAGTATCATTTTTG-3'
Reverse sequence:
5'-AATTCAAAAATGATACTAGACAGCTACAAATCTCGAGATTTGTAGCTGTCTAGTATCA-3'

Other clones with same target sequence:

(none)