Construct: shRNA TRCN0000430002

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_153028.2-1735s21c1
DNA Barcode:
CAGTAGCAGGCTTACCAATTT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
ZNF75A (7627)
Gene Description:
zinc finger protein 75a
Transcript:
RefSeq NM_153028.2 (NON-CURRENT)
Match location:
Position 1735 (3UTR)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000430002)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CAGTAGCAGGCTTACCAATTT
Hairpin Sequence:
5'-CCGG-CAGTAGCAGGCTTACCAATTT-CTCGAG-AAATTGGTAAGCCTGCTACTG-TTTTTTGAAT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCAGTAGCAGGCTTACCAATTTCTCGAGAAATTGGTAAGCCTGCTACTGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACAGTAGCAGGCTTACCAATTTCTCGAGAAATTGGTAAGCCTGCTACTG-3'

Other clones with same target sequence:

(none)