Construct: shRNA TRCN0000430009

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_053044.2-1344s21c1
DNA Barcode:
AGAGGTCAGCAGTGGAATTTA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
HTRA3 (94031)
Gene Description:
HtrA serine peptidase 3
Transcript:
RefSeq NM_053044.2 (NON-CURRENT)
Match location:
Position 1344 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000430009)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
AGAGGTCAGCAGTGGAATTTA
Hairpin Sequence:
5'-CCGG-AGAGGTCAGCAGTGGAATTTA-CTCGAG-TAAATTCCACTGCTGACCTCT-TTTTTTGAAT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGAGAGGTCAGCAGTGGAATTTACTCGAGTAAATTCCACTGCTGACCTCTTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAAGAGGTCAGCAGTGGAATTTACTCGAGTAAATTCCACTGCTGACCTCT-3'

Other clones with same target sequence:

(none)