Construct: shRNA TRCN0000430031

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_016673.2-773s21c1
DNA Barcode:
ACACTACGGCCATCACCTTTG

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Cntfr (12804)
Gene Description:
ciliary neurotrophic factor receptor
Transcript:
RefSeq NM_016673.2 (CURRENT)
Match location:
Position 773 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000430031)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
ACACTACGGCCATCACCTTTG
Hairpin Sequence:
5'-CCGG-ACACTACGGCCATCACCTTTG-CTCGAG-CAAAGGTGATGGCCGTAGTGT-TTTTTTGAAT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGACACTACGGCCATCACCTTTGCTCGAGCAAAGGTGATGGCCGTAGTGTTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAACACTACGGCCATCACCTTTGCTCGAGCAAAGGTGATGGCCGTAGTGT-3'

Other clones with same target sequence:

(none)