Construct: shRNA TRCN0000430049

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_005250.2-1452s21c1
DNA Barcode:
GAGCAGCTACGGAGACTTAAA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
FOXL1 (2300)
Gene Description:
forkhead box L1
Transcript:
RefSeq NM_005250.2 (NON-CURRENT)
Match location:
Position 1452 (3UTR)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000430049)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GAGCAGCTACGGAGACTTAAA
Hairpin Sequence:
5'-CCGG-GAGCAGCTACGGAGACTTAAA-CTCGAG-TTTAAGTCTCCGTAGCTGCTC-TTTTTTGAAT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGAGCAGCTACGGAGACTTAAACTCGAGTTTAAGTCTCCGTAGCTGCTCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGAGCAGCTACGGAGACTTAAACTCGAGTTTAAGTCTCCGTAGCTGCTC-3'

Other clones with same target sequence:

(none)