Construct: shRNA TRCN0000430082

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_144579.2-799s21c1
DNA Barcode:
AGCTGGAGGAAGGGATTGATG

Original Target:

Taxon:
Homo sapiens (human)
Gene:
SFXN5 (94097)
Gene Description:
sideroflexin 5
Transcript:
RefSeq NM_144579.2 (NON-CURRENT)
Match location:
Position 799 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000430082)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
AGCTGGAGGAAGGGATTGATG
Hairpin Sequence:
5'-CCGG-AGCTGGAGGAAGGGATTGATG-CTCGAG-CATCAATCCCTTCCTCCAGCT-TTTTTGGAAT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGAGCTGGAGGAAGGGATTGATGCTCGAGCATCAATCCCTTCCTCCAGCTTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAAGCTGGAGGAAGGGATTGATGCTCGAGCATCAATCCCTTCCTCCAGCT-3'

Other clones with same target sequence:

(none)