Construct: shRNA TRCN0000430088

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_003516.2-394s21c1
DNA Barcode:
CCGTACTGCTCCCTAAGAAGA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
H2AC18 (8337)
Gene Description:
H2A clustered histone 18
Transcript:
RefSeq NM_003516.2 (CURRENT)
Match location:
Position 394 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000430088)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCGTACTGCTCCCTAAGAAGA
Hairpin Sequence:
5'-CCGG-CCGTACTGCTCCCTAAGAAGA-CTCGAG-TCTTCTTAGGGAGCAGTACGG-TTTTTTGAAT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCGTACTGCTCCCTAAGAAGACTCGAGTCTTCTTAGGGAGCAGTACGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCGTACTGCTCCCTAAGAAGACTCGAGTCTTCTTAGGGAGCAGTACGG-3'

Other clones with same target sequence:

(none)