Construct: shRNA TRCN0000430168

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_002785.2-1300s21c1
DNA Barcode:
TCCATTTCATTGGACTAAATA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
PSG11 (5680)
Gene Description:
pregnancy specific beta-1-glycoprotein 11
Transcript:
RefSeq NM_002785.2 (NON-CURRENT)
Match location:
Position 1300 (3UTR)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000430168)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
TCCATTTCATTGGACTAAATA
Hairpin Sequence:
5'-CCGC-TCCATTTCATTGGACTAAATA-CTCGAG-TATTTAGTCCAATGAAATGGA-TTTTTTGAAT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGTCCATTTCATTGGACTAAATACTCGAGTATTTAGTCCAATGAAATGGATTTTTG-3'
Reverse sequence:
5'-AATTCAAAAATCCATTTCATTGGACTAAATACTCGAGTATTTAGTCCAATGAAATGGA-3'

Other clones with same target sequence:

(none)