Construct: shRNA TRCN0000430180

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_014211.2-825s21c1
DNA Barcode:
GTACACCATAGAGCGGTATTT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
GABRP (2568)
Gene Description:
gamma-aminobutyric acid type A receptor pi subunit
Transcript:
RefSeq NM_014211.2 (NON-CURRENT)
Match location:
Position 825 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000430180)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GTACACCATAGAGCGGTATTT
Hairpin Sequence:
5'-CCGG-GTACACCATAGAGCGGTATTT-CTCGAG-AAATACCGCTCTATGGTGTAC-TTTTTTGAAT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGTACACCATAGAGCGGTATTTCTCGAGAAATACCGCTCTATGGTGTACTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGTACACCATAGAGCGGTATTTCTCGAGAAATACCGCTCTATGGTGTAC-3'

Other clones with same target sequence:

(none)