Construct: shRNA TRCN0000430221

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_000811.2-1060s21c1
DNA Barcode:
GCTACTTCATGATACAGATAT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
GABRA6 (2559)
Gene Description:
gamma-aminobutyric acid type A receptor alpha6 subunit
Transcript:
RefSeq NM_000811.2 (NON-CURRENT)
Match location:
Position 1060 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000430221)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCTACTTCATGATACAGATAT
Hairpin Sequence:
5'-CCGG-GCTACTTCATGATACAGATAT-CTCGAG-ATATCTGTATCATGAAGTAGC-TTTTTTGAAT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCTACTTCATGATACAGATATCTCGAGATATCTGTATCATGAAGTAGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCTACTTCATGATACAGATATCTCGAGATATCTGTATCATGAAGTAGC-3'

Other clones with same target sequence:

(none)