Construct: shRNA TRCN0000430226

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_020845.2-1354s21c1
DNA Barcode:
CATCATAGAGGACGAGGTTAG

Original Target:

Taxon:
Homo sapiens (human)
Gene:
PITPNM2 (57605)
Gene Description:
phosphatidylinositol transfer protein membrane associated 2
Transcript:
RefSeq NM_020845.2 (CURRENT)
Match location:
Position 1354 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000430226)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CATCATAGAGGACGAGGTTAG
Hairpin Sequence:
5'-CCGG-CATCATAGAGGACGAGGTTAG-CTCGAG-CTAACCTCGTCCTCTATGATG-TTTTTTGAAT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCATCATAGAGGACGAGGTTAGCTCGAGCTAACCTCGTCCTCTATGATGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACATCATAGAGGACGAGGTTAGCTCGAGCTAACCTCGTCCTCTATGATG-3'

Other clones with same target sequence:

(none)