Construct: shRNA TRCN0000430265

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_001519.2-1306s21c1
DNA Barcode:
GAGGAGGTTGAAGGTGAAATA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
BRF1 (2972)
Gene Description:
BRF1 RNA polymerase III transcription initiation factor subunit
Transcript:
RefSeq NM_001519.2 (NON-CURRENT)
Match location:
Position 1306 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000430265)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GAGGAGGTTGAAGGTGAAATA
Hairpin Sequence:
5'-CCGG-GAGGAGGTTGAAGGTGAAATA-CTCGAG-TATTTCACCTTCAACCTCCTC-TTTTTTGAAT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGAGGAGGTTGAAGGTGAAATACTCGAGTATTTCACCTTCAACCTCCTCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGAGGAGGTTGAAGGTGAAATACTCGAGTATTTCACCTTCAACCTCCTC-3'

Other clones with same target sequence:

(none)