Construct: shRNA TRCN0000430284

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_012095.4-1078s21c1
DNA Barcode:
ATGGAAATTTCCGACTCATAT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
AP3M1 (26985)
Gene Description:
adaptor related protein complex 3 subunit mu 1
Transcript:
RefSeq NM_012095.4 (NON-CURRENT)
Match location:
Position 1078 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000430284)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
ATGGAAATTTCCGACTCATAT
Hairpin Sequence:
5'-CCGG-ATGGAAATTTCCGACTCATAT-CTCGAG-ATATGAGTCGGAAATTTCCAT-TTTTTTGAAT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGATGGAAATTTCCGACTCATATCTCGAGATATGAGTCGGAAATTTCCATTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAATGGAAATTTCCGACTCATATCTCGAGATATGAGTCGGAAATTTCCAT-3'

Other clones with same target sequence:

(none)