Construct: shRNA TRCN0000430306

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_183374.2-1272s21c1
DNA Barcode:
TCCCGAAGGCTTCGATCCAGA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
CYP26C1 (340665)
Gene Description:
cytochrome P450 family 26 subfamily C member 1
Transcript:
RefSeq NM_183374.2 (NON-CURRENT)
Match location:
Position 1272 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000430306)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
TCCCGAAGGCTTCGATCCAGA
Hairpin Sequence:
5'-CCGG-TCCCGAAGGCTTCGATCCAGA-CTCGAG-TCTGGATCGAAGCCTTCGGGA-TTTTTTGAAT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGTCCCGAAGGCTTCGATCCAGACTCGAGTCTGGATCGAAGCCTTCGGGATTTTTG-3'
Reverse sequence:
5'-AATTCAAAAATCCCGAAGGCTTCGATCCAGACTCGAGTCTGGATCGAAGCCTTCGGGA-3'

Other clones with same target sequence:

(none)