Construct: shRNA TRCN0000430359

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_024943.1-634s21c1
DNA Barcode:
AGCAAGGAGAAATCGATAAAC

Original Target:

Taxon:
Homo sapiens (human)
Gene:
TMEM156 (80008)
Gene Description:
transmembrane protein 156
Transcript:
RefSeq NM_024943.1 (NON-CURRENT)
Match location:
Position 634 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000430359)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
AGCAAGGAGAAATCGATAAAC
Hairpin Sequence:
5'-CCGG-AGCAAGGAGAAATCGATAAAC-CTCGAG-GTTTATCGATTTCTCCTTGCT-TTTTTTGAAT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGAGCAAGGAGAAATCGATAAACCTCGAGGTTTATCGATTTCTCCTTGCTTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAAGCAAGGAGAAATCGATAAACCTCGAGGTTTATCGATTTCTCCTTGCT-3'

Other clones with same target sequence:

(none)