Construct: shRNA TRCN0000430360

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_001771.2-2445s21c1
DNA Barcode:
TGCGATGACACGGTCACTTAT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
CD22 (933)
Gene Description:
CD22 molecule
Transcript:
RefSeq NM_001771.2 (NON-CURRENT)
Match location:
Position 2445 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000430360)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
TGCGATGACACGGTCACTTAT
Hairpin Sequence:
5'-CCGG-TGCGATGACACGGTCACTTAT-CTCGAG-ATAAGTGACCGTGTCATCGCA-TTTTTTGAAT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGTGCGATGACACGGTCACTTATCTCGAGATAAGTGACCGTGTCATCGCATTTTTG-3'
Reverse sequence:
5'-AATTCAAAAATGCGATGACACGGTCACTTATCTCGAGATAAGTGACCGTGTCATCGCA-3'

Other clones with same target sequence:

(none)