Construct: shRNA TRCN0000430400

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_018960.4-518s21c1
DNA Barcode:
GCCTACTGGTCATTGATCATC

Original Target:

Taxon:
Homo sapiens (human)
Gene:
GNMT (27232)
Gene Description:
glycine N-methyltransferase
Transcript:
RefSeq NM_018960.4 (NON-CURRENT)
Match location:
Position 518 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000430400)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCCTACTGGTCATTGATCATC
Hairpin Sequence:
5'-CCGG-GCCTACTGGTCATTGATCATC-CTCGAG-GATGATCAATGACCAGTAGGC-TTTTTTGAAT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCCTACTGGTCATTGATCATCCTCGAGGATGATCAATGACCAGTAGGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCCTACTGGTCATTGATCATCCTCGAGGATGATCAATGACCAGTAGGC-3'

Other clones with same target sequence:

(none)