Construct: shRNA TRCN0000430408

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_005342.2-185s21c1
DNA Barcode:
CCCAGAGGTCCCTGTCAATTT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
HMGB3 (3149)
Gene Description:
high mobility group box 3
Transcript:
RefSeq NM_005342.2 (NON-CURRENT)
Match location:
Position 185 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000430408)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCCAGAGGTCCCTGTCAATTT
Hairpin Sequence:
5'-CCGG-CCCAGAGGTCCCTGTCAATTT-CTCGAG-AAATTGACAGGGACCTCTGGG-TTTTTTGAAT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCCAGAGGTCCCTGTCAATTTCTCGAGAAATTGACAGGGACCTCTGGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCCAGAGGTCCCTGTCAATTTCTCGAGAAATTGACAGGGACCTCTGGG-3'

Other clones with same target sequence:

(none)