Construct: shRNA TRCN0000430518

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_001009555.3-2817s21c1
DNA Barcode:
GTGTTGCTCGGTTTGAATATA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
SH3D19 (152503)
Gene Description:
SH3 domain containing 19
Transcript:
RefSeq NM_001009555.3 (CURRENT)
Match location:
Position 2817 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000430518)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GTGTTGCTCGGTTTGAATATA
Hairpin Sequence:
5'-CCGG-GTGTTGCTCGGTTTGAATATA-CTCGAG-TATATTCAAACCGAGCAACAC-TTTTTTGAAT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGTGTTGCTCGGTTTGAATATACTCGAGTATATTCAAACCGAGCAACACTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGTGTTGCTCGGTTTGAATATACTCGAGTATATTCAAACCGAGCAACAC-3'

Other clones with same target sequence:

(none)