Construct: shRNA TRCN0000430529
Construct Description:
- Construct Type:
- shRNA
- Other Identifiers:
- NM_018667.3-2724s21c1
- DNA Barcode:
- GCTTCGTCCAGGGTTCTTTAT
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- SMPD3 (55512)
- Gene Description:
- sphingomyelin phosphodiesterase 3
- Transcript:
- RefSeq NM_018667.3 (NON-CURRENT)
- Match location:
- Position 2724 (3UTR)
Vector Information:
- Vector Backbone:
- pLKO_005
- Pol II Cassette 1:
- PGK-PuroR
- Pol II Cassette 2:
- n/a
- Pol III Promoter:
- constitutive hU6
- Pol III Insert:
- (TRCN0000430529)
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
Current transcripts matched by this shRNA:
Sequence Information
- Target Sequence:
- GCTTCGTCCAGGGTTCTTTAT
- Hairpin Sequence:
-
5'-CCGG-GCTTCGTCCAGGGTTCTTTAT-CTCGAG-ATAAAGAACCCTGGACGAAGC-TTTTTTGAAT-3'
Oligo design for arrayed cloning:
- Forward sequence:
- 5'-CCGGGCTTCGTCCAGGGTTCTTTATCTCGAGATAAAGAACCCTGGACGAAGCTTTTTG-3'
- Reverse sequence:
- 5'-AATTCAAAAAGCTTCGTCCAGGGTTCTTTATCTCGAGATAAAGAACCCTGGACGAAGC-3'
Other clones with same target sequence:
(none)
