Construct: shRNA TRCN0000430558

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_002581.3-3125s21c1
DNA Barcode:
TTGGCAGTGTGTACCAGTATT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
PAPPA (5069)
Gene Description:
pappalysin 1
Transcript:
RefSeq NM_002581.3 (NON-CURRENT)
Match location:
Position 3125 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000430558)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
TTGGCAGTGTGTACCAGTATT
Hairpin Sequence:
5'-CCGG-TTGGCAGTGTGTACCAGTATT-CTCGAG-AATACTGGTACACACTGCCAA-TTTTTTGAAT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGTTGGCAGTGTGTACCAGTATTCTCGAGAATACTGGTACACACTGCCAATTTTTG-3'
Reverse sequence:
5'-AATTCAAAAATTGGCAGTGTGTACCAGTATTCTCGAGAATACTGGTACACACTGCCAA-3'

Other clones with same target sequence:

(none)