Construct: shRNA TRCN0000430597

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_003428.4-714s21c1
DNA Barcode:
GATAACCAAGACAAGCTTAAA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
ZNF84 (7637)
Gene Description:
zinc finger protein 84
Transcript:
RefSeq NM_003428.4 (NON-CURRENT)
Match location:
Position 714 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000430597)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GATAACCAAGACAAGCTTAAA
Hairpin Sequence:
5'-CCGG-GATAACCAAGACAAGCTTAAA-CTCGAG-TTTAAGCTTGTCTTGGTTATC-TTTTTTGAAT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGATAACCAAGACAAGCTTAAACTCGAGTTTAAGCTTGTCTTGGTTATCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGATAACCAAGACAAGCTTAAACTCGAGTTTAAGCTTGTCTTGGTTATC-3'

Other clones with same target sequence:

(none)