Construct: shRNA TRCN0000430731

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_080660.3-1197s21c1
DNA Barcode:
CACTTAATATTGTCTCATAAG

Original Target:

Taxon:
Homo sapiens (human)
Gene:
ZC3HAV1L (92092)
Gene Description:
zinc finger CCCH-type containing, antiviral 1 like
Transcript:
RefSeq NM_080660.3 (NON-CURRENT)
Match location:
Position 1197 (3UTR)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000430731)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CACTTAATATTGTCTCATAAG
Hairpin Sequence:
5'-CCGG-CACTTAATATTGTCTCATAAG-CTCGAG-CTTATGAGACAATATTAAGTG-TTTTTTGAAT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCACTTAATATTGTCTCATAAGCTCGAGCTTATGAGACAATATTAAGTGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACACTTAATATTGTCTCATAAGCTCGAGCTTATGAGACAATATTAAGTG-3'

Other clones with same target sequence:

(none)