Construct: shRNA TRCN0000430738

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_018124.3-1689s21c1
DNA Barcode:
ACCGTGGTCCAGACTTATAAT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
RFWD3 (55159)
Gene Description:
ring finger and WD repeat domain 3
Transcript:
RefSeq NM_018124.3 (NON-CURRENT)
Match location:
Position 1689 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000430738)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
ACCGTGGTCCAGACTTATAAT
Hairpin Sequence:
5'-CCGG-ACCGTGGTCCAGACTTATAAT-CTCGAG-ATTATAAGTCTGGACCACGGT-TTTTTTGAAT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGACCGTGGTCCAGACTTATAATCTCGAGATTATAAGTCTGGACCACGGTTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAACCGTGGTCCAGACTTATAATCTCGAGATTATAAGTCTGGACCACGGT-3'

Other clones with same target sequence:

(none)