Construct: shRNA TRCN0000430795

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_004607.2-392s21c1
DNA Barcode:
AGCACGTTTAGTACTGGATTC

Original Target:

Taxon:
Homo sapiens (human)
Gene:
TBCA (6902)
Gene Description:
tubulin folding cofactor A
Transcript:
RefSeq NM_004607.2 (CURRENT)
Match location:
Position 392 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000430795)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
AGCACGTTTAGTACTGGATTC
Hairpin Sequence:
5'-CCGG-AGCACGTTTAGTACTGGATTC-CTCGAG-GAATCCAGTACTAAACGTGCT-TTTTTTGAAT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGAGCACGTTTAGTACTGGATTCCTCGAGGAATCCAGTACTAAACGTGCTTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAAGCACGTTTAGTACTGGATTCCTCGAGGAATCCAGTACTAAACGTGCT-3'

Other clones with same target sequence:

(none)