Construct: shRNA TRCN0000430845

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_152667.2-1288s21c1
DNA Barcode:
ACATGCAAATCAAGGTATTAT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
NANP (140838)
Gene Description:
N-acetylneuraminic acid phosphatase
Transcript:
RefSeq NM_152667.2 (NON-CURRENT)
Match location:
Position 1288 (3UTR)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000430845)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
ACATGCAAATCAAGGTATTAT
Hairpin Sequence:
5'-CCGG-ACATGCAAATCAAGGTATTAT-CTCGAG-ATAATACCTTGATTTGCATGT-TTTTTTGAAT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGACATGCAAATCAAGGTATTATCTCGAGATAATACCTTGATTTGCATGTTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAACATGCAAATCAAGGTATTATCTCGAGATAATACCTTGATTTGCATGT-3'

Other clones with same target sequence:

(none)