Construct: shRNA TRCN0000430972

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_033083.6-1029s21c1
DNA Barcode:
AGTGCTGGATCTTTCGAAACC

Original Target:

Taxon:
Homo sapiens (human)
Gene:
EAF1 (85403)
Gene Description:
ELL associated factor 1
Transcript:
RefSeq NM_033083.6 (NON-CURRENT)
Match location:
Position 1029 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000430972)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
AGTGCTGGATCTTTCGAAACC
Hairpin Sequence:
5'-CCGG-AGTGCTGGATCTTTCGAAACC-CTCGAG-GGTTTCGAAAGATCCAGCACT-TTTTTTGAAT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGAGTGCTGGATCTTTCGAAACCCTCGAGGGTTTCGAAAGATCCAGCACTTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAAGTGCTGGATCTTTCGAAACCCTCGAGGGTTTCGAAAGATCCAGCACT-3'

Other clones with same target sequence:

(none)